For informational and research purposes only.
For informational and research purposes only.
Free calculation tool
Calculate peptide concentration, mg or mcg per dose, and mL or U-100 syringe units. Choose guided help from PEP or quick advanced inputs, including whole-spray nasal calculations.
Meet PEP. Six small steps, with the why behind every number.
Step 1 of 6
PEP & step guideHave a COA? Look for a measured amount per vial for this product and batch. Otherwise, use the label as an estimate.
PEP explains
First check a verified COA for this product and batch. If it reports a measured amount per vial, use that for your educational estimate. Without it, identify the label as an estimate.
More peptide than the number entered means a higher dose in the same liquid volume; less means a lower dose. PEP can’t measure what’s inside. For a prescribed medication, ask your pharmacist to resolve differences before changing the calculation.
COA means Certificate of Analysis, a lab report. Check that it is authentic and matches the product and batch (lot number). Look for the measured amount of the intended compound per vial, with units such as mg/vial—not just powder weight, a batch total, or “99% purity.” Purity and amount are different measurements. If the report gives only percentages or the meaning is unclear, ask the lab to explain the amount per vial. A batch result does not measure every vial. For a prescribed medication, have your pharmacist resolve any difference from the label before changing the calculation.
mg and mcg measure the amount of peptide or medication: 1 mg = 1,000 mcg. IU measures how much a substance acts in the body. There is no single way to convert IU to mg for every product. Use the unit printed on your label. A syringe’s U-100 units measure liquid instead.
More peptide means a higher dose in the same amount of liquid; less means a lower dose. Example: 10 mg in 2 mL gives 5 mg/mL, but 12 mg in 2 mL gives 6 mg/mL. The same 0.1 mL would contain 0.5 mg or 0.6 mg. Use a matching, measured fill result when available for an educational estimate. Do not invent an overfill correction or judge the amount from powder appearance.
mg/mL means the amount in each mL. For example, 2 mL of liquid at 2.5 mg/mL contains 5 mg in total. Enter 5 mg and 2 mL. Use the actual liquid volume, not the bottle’s capacity. Extra liquid at the same concentration does not make it stronger. An already mixed product may need no added liquid; check its instructions.
The fill amount and target dose must describe the same ingredient or the same total blend. A total blend weight does not tell you each ingredient’s amount. Do not use the combined weight to calculate a dose of just one ingredient; ask your pharmacist for the component amounts.
Educational reference only
Always verify these inputs and results with your pharmacist, prescriber, or healthcare professional before use.
The fill amount and solution volume determine the concentration—and how much liquid contains your target dose.
The amount of peptide in the vial, measured in milligrams (mg) or micrograms (mcg). This describes the contents, not the size of the bottle.
1 mg = 1,000 mcg
The total volume of liquid the peptide is dissolved in after mixing. If you use more than one diluent, count all of them together.
Measured in milliliters (mL), not bottle capacity.
The amount of peptide in each milliliter of solution. A higher concentration means less liquid is needed for the same target dose; a lower concentration means more.
Fill amount (mg) ÷ solution volume (mL) = concentration (mg/mL).
Direct answers about the math, the units, and the limits of a calculator.
Reconstitution means dissolving powder in a suitable liquid. The total amount and the liquid volume after mixing tell you how much is in each mL. Follow your product’s instructions for the liquid, volume, and preparation.
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For an educational calculation, first check a verified Certificate of Analysis (COA) for the same product and batch. If it reports a measured amount of the intended compound per vial, use that amount. A purity percentage alone is not a fill amount. Without a matching measured result, identify the label as an estimate. A batch result does not measure every vial. For a prescribed medication, ask your pharmacist to resolve any difference from the label before changing the calculation.
These are common label and preset amounts, so PEP asks you to check the source. A round number can be correct, and a decimal does not prove that an amount was tested. Choose a matching COA with a measured amount per vial, the label as an estimate, or a learning example. PEP uses the number you enter without adding an overfill correction. More actual peptide means a higher dose in the same liquid volume; less means a lower dose.
Divide the total amount in the vial by the liquid volume after mixing. For example, 5 mg in 2 mL gives 2.5 mg in each mL, written as 2.5 mg/mL. That is also 2,500 mcg/mL. PEP shows the concentration in mg/mL even if you entered mcg.
1 mg = 1,000 mcg. Divide mcg by 1,000 to get mg: 250 mcg = 0.25 mg. PEP does this conversion for you.
Divide the target dose by the amount in each mL. First put both amounts in the same unit. For example, 250 mcg = 0.25 mg. At 2.5 mg/mL, 0.25 ÷ 2.5 = 0.1 mL of liquid. Your prescriber chooses the dose; this calculation finds the liquid volume.
On a U-100 syringe, 1 mL corresponds to 100 units, so 10 units = 0.1 mL and 1 unit = 0.01 mL. Check the syringe’s actual scale. U-40 and U-500 scales use different conversions and are not supported by this calculator.
No. U-100 syringe units measure liquid volume. Medication IU (International Units) measure how much a substance acts in the body. There is no single IU-to-mg conversion for every product. If your product uses IU, enter both the vial amount and target dose in IU. PEP calculates the volume in mL.
It depends on concentration. On a U-100 syringe, 10 units = 0.1 mL. At 2.5 mg/mL that volume contains 0.25 mg; at 5 mg/mL it contains 0.5 mg. A unit marking alone cannot tell you the amount of medication.
Use the volume in your product’s instructions. There is no single amount that works for every product. Adding more liquid puts less peptide in each mL, so you need more liquid for the same dose. The comparison tool shows that math; it cannot decide which mixture is suitable.
Bacteriostatic water is sterile water with a preservative, such as benzyl alcohol. The preservative limits bacterial growth; it does not make contaminated liquid free of germs. Check the product’s instructions before choosing it.
Only if your product’s instructions allow it. Sterile water has no preservative; bacteriostatic water contains one; saline is salt water. They do not work the same way with every product. The calculator uses their volumes, but cannot tell you whether a substitute is suitable.
Yes. In Guided mode, choose Multiple diluents at the liquid step. In Advanced mode, find it under More options. You can enter up to three liquids, and PEP adds their volumes. Only combine liquids if your product’s instructions allow it.
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No. mL measures space for liquid; mg measures the amount of peptide or medication. A 3 mL bottle can contain 5 mg, 10 mg, or another amount. Enter the capacity and Fill Amount separately. PEP warns you if the liquid volume is larger than the capacity you entered.
Not always. The powder and other ingredients can change the total volume after mixing. PEP assumes the liquid volumes you enter equal that total. If your product gives a different final volume or concentration, use that information.
Follow the product’s written storage instructions and discard date. There is no rule that every mixed peptide lasts the same number of days. In particular, a preservative does not mean every mixture lasts 28 days. Ask your pharmacist if the instructions are unclear.
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Do not use it if you are unsure it is free of germs. Clear liquid can still be contaminated, even if it contains a preservative. Ask your pharmacist how to handle and discard it. Use a new sterile needle and syringe for each vial entry.
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The calculator works backward from your fill amount and target dose to look for simple concentrations and measurable draws. It rounds suggested mixing volumes to 0.01 mL, then recalculates the actual concentration. Selecting an injection option also shows a rounded draw and its estimated dose difference. Without a selected option, results stay exact apart from display rounding. Confirm both liquid measurement and dose differences with your pharmacist.
Do not assume the routes are interchangeable. Nasal formulation, pump performance, and absorption differ from injection. This calculator estimates concentration and the amount emitted by a full spray; it cannot determine an equivalent nasal dose, compatible ingredients, or how much is absorbed. Use product-specific instructions confirmed by your pharmacist or prescriber.
It means one full pump emits a nominal 0.1 mL, or 100 microliters (µL). As a math example, 5 mg/mL × 0.1 mL = 0.5 mg per spray; a 1 mg target would be two full sprays. This is not a dose recommendation. Check the actual device specification and priming instructions. A partial pump is not a reliable half-dose.
Choose the size of your actual syringe: 0.3, 0.5, 1, or 3 mL. The calculated liquid volume must fit within that capacity. Check the lines too: each line may mark a different amount on different syringes. Ask your pharmacist which device can measure your dose accurately.
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Rounding changes the dose. For example, 0.5 U-100 unit is 0.005 mL. Rounding up to 1 unit doubles the liquid and the dose. PEP keeps the exact draw until you select a liquid suggestion with an explicitly rounded draw. The rounding details show the dose difference; PEP also checks the illustrated markings. Ask your pharmacist how to measure the result accurately.
Divide the total amount by one dose, using the same units. For example, 5 mg ÷ 0.25 mg = 20 doses. Liquid left in the vial, needle, or syringe can mean fewer full doses. A decimal means part of another dose. Follow the storage and discard instructions.
PEP multiplies the amount per mL by the liquid volume from one full pump. That gives the amount per spray. It then divides your target dose by that amount. Use your device’s stated volume and preparation instructions. A partial pump is not a reliable dose. Your prescriber must confirm whether nasal use is suitable.
Only if the vial amount and target dose describe the same ingredient or the same total mixture. Knowing the total weight of a blend does not tell you how much of each ingredient is inside. PEP cannot calculate separate doses for the ingredients in a blend.